Peptide Reconstitution Calculator

A peptide dosage calculator removes the guesswork from reconstitution math. When you dissolve lyophilized powder in bacteriostatic water, the concentration of the resulting solution depends on two numbers: the mass of peptide in the vial and the volume of water you add. We built the calculator below to handle that arithmetic for you. Enter your vial size and water volume, and it returns the concentration per milliliter and per 0.1 ml tick mark on a standard insulin syringe.

How Reconstitution Math Works

The Core Formula

Concentration equals mass divided by volume. That is it. If you have a 10 mg vial and add 2 ml of bacteriostatic water, you get 5 mg per ml, which is 5000 mcg per ml, or 500 mcg per 0.1 ml. Each tick on a 1 ml insulin syringe represents 0.1 ml, so each tick delivers 500 mcg. We use this formula throughout the site for every product where the vial size is disclosed.

Step-by-Step Conversion

Start with the peptide amount in milligrams. Multiply by 1000 to convert to micrograms. Divide by the total water volume in milliliters. That gives you mcg per ml. Then multiply by 0.1 to get the amount per tick mark. The formula written out:

mcg per 0.1 ml = (peptide mg x 1000) / water ml x 0.1

Step-by-Step Reconstitution Process

1. Gather your supplies You will need the lyophilized peptide vial, bacteriostatic water, alcohol swabs, and an insulin syringe (1 ml, 29-31 gauge)
2. Swab both stoppers Wipe the rubber stopper on both the peptide vial and the bacteriostatic water vial with an alcohol swab. Let each dry for about 10 seconds
3. Draw the water Pull the desired volume of bacteriostatic water into the syringe using the calculator result above to determine how much
4. Add water slowly along the glass wall Insert the needle into the peptide vial at an angle. Release the water so it runs down the inside wall of the vial rather than hitting the powder directly
5. Swirl gently to dissolve Tilt and rotate the vial. Do not shake. The powder should dissolve into a clear solution within 1 to 2 minutes
6. Label, date and refrigerate Write the peptide name, concentration and reconstitution date on the vial. Store at 2-8 C immediately

Common Vial Sizes and Concentrations

The table below shows concentration values for the most frequently used vial and water combinations. All figures assume complete dissolution with no losses during the reconstitution process.

Supplies You Will Need

Bacteriostatic Water

Bacteriostatic water contains 0.9% benzyl alcohol, which inhibits microbial growth and allows you to draw from the same vial multiple times over several weeks. We list Real Peptides Bacteriostatic Water at $4.80 for a 10 ml vial. Standard peptide reconstitution calls for 1 to 2.5 ml per vial, so a single 10 ml bottle covers multiple reconstitutions. See our Real Peptides Bacteriostatic Water product page for full details.

Insulin Syringes

A 1 ml insulin syringe with a 29 to 31 gauge needle is the standard tool for both reconstitution and drawing from the reconstituted vial. The 0.1 ml graduations printed on the syringe barrel correspond directly to the concentration figures from our calculator.

Alcohol Swabs

We recommend individually wrapped 70% isopropyl alcohol swabs for wiping rubber stoppers. This step reduces the risk of introducing contaminants into the solution during reconstitution.